Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| E. coliDH5α | 39 | |
| P. syringae pv. glycinea | ||
| PG4180.N9 | COR+; Kmr | 44 |
| PG4180.P2 | COR−;corR::Gmr | 35 |
| Plasmids | ||
| pAP06.415 | AprTcr; contains corR; chimeric plasmid constructed from pAP06 and pRK415; 17.7 kb | 35 |
| pAP06.ΔC | Apr Tcr; a 242-bp deletion was introduced into the C terminus of corR; derived from pAP06.415 | This study |
| pMAL-c2 | Apr; ColE1 origin,tac promoter, encodes malE andlacZα; contains factor Xa cleavage site | New England Biolabs |
| pRK415 | Tcr; RK2-derived cloning vector | 18 |
| pBluescript SK(+) | Apr; ColE1 origin, cloning vehicle | Stratagene |
| pRG960sd | Smr Spr; contains promoterless uidA with start codon and Shine-Dalgarno sequence | 46 |
| pHLP1 | SmrSpr; 0.46-kb XbaI-PstI fragment from pHL1 in pRG960sd (XbaI-PstI-uidA) | 24 |
| pHLP3 | Smr Spr; 1.4-kbSalI-PstI fragment from pHL1 in pRG960sd | 23 |
| pBBR1MCS | Cmr; 4.7-kb broad-host-range cloning vector | 20 |
| pCFLP | Cmr; 8-kb promoter probe vector containing the cfl promoter as a PstI-SalI fragment and the uidA gene from pHLP3 in pBBR1MCS | This study |
| pCFLPΔ433 | Cmr; derivative of pCFLP containing a 433-bp deletion from the 5′ end (SalI site) of the cfl promoter | This study |
| pCFLPΔ726 | Cmr; derivative of pCFLP containing a 726-bp deletion from the 5′ end (SalI site) of thecfl promoter | This study |
| pBBR.Gus | Cmr; 6.6-kb promoter probe vector containing the uidA gene from pHLP3 in pBBR1MCS | This study |
| pAPXS9 | Apr; 950-bpXbaI-SalI fragment from pHLP3 in pBluescript SK(+) | This study |
| pAPSS27 | Apr; 278-bpStyI-ScaI fragment from pAPXS9 in pBluescript SK(+) | This study |
| pAPSB36 | Apr; 360-bpStyI-BsrBI fragment from pAPXS9 in pBluescript SK(+) | This study |