Table 1.

Bacterial strains and plasmids

Strain or plasmidRelevant characteristicsReference or source
E. coliDH5α 39
P. syringae pv. glycinea
 PG4180.N9COR+; Kmr 44
 PG4180.P2COR;corR::Gmr 35
Plasmids
 pAP06.415AprTcr; contains corR; chimeric plasmid constructed from pAP06 and pRK415; 17.7 kb 35
 pAP06.ΔCApr Tcr; a 242-bp deletion was introduced into the C terminus of corR; derived from pAP06.415This study
 pMAL-c2Apr; ColE1 origin,tac promoter, encodes malE andlacZα; contains factor Xa cleavage siteNew England Biolabs
 pRK415Tcr; RK2-derived cloning vector 18
 pBluescript SK(+)Apr; ColE1 origin, cloning vehicleStratagene
 pRG960sdSmr Spr; contains promoterless uidA with start codon and Shine-Dalgarno sequence 46
 pHLP1SmrSpr; 0.46-kb XbaI-PstI fragment from pHL1 in pRG960sd (XbaI-PstI-uidA) 24
 pHLP3Smr Spr; 1.4-kbSalI-PstI fragment from pHL1 in pRG960sd 23
 pBBR1MCSCmr; 4.7-kb broad-host-range cloning vector 20
 pCFLPCmr; 8-kb promoter probe vector containing the cfl promoter as a PstI-SalI fragment and the uidA gene from pHLP3 in pBBR1MCSThis study
 pCFLPΔ433Cmr; derivative of pCFLP containing a 433-bp deletion from the 5′ end (SalI site) of the cfl promoterThis study
 pCFLPΔ726Cmr; derivative of pCFLP containing a 726-bp deletion from the 5′ end (SalI site) of thecfl promoterThis study
 pBBR.GusCmr; 6.6-kb promoter probe vector containing the uidA gene from pHLP3 in pBBR1MCSThis study
 pAPXS9Apr; 950-bpXbaI-SalI fragment from pHLP3 in pBluescript SK(+)This study
 pAPSS27Apr; 278-bpStyI-ScaI fragment from pAPXS9 in pBluescript SK(+)This study
 pAPSB36Apr; 360-bpStyI-BsrBI fragment from pAPXS9 in pBluescript SK(+)This study