Table 1.

Effects of systematic single substitutions in the TTGN11CAA sequence on the formation of retarded complexes of the R. etli recA promotera

Operator sequenceNucleotide substitutionBinding activity (%)
Wild type
TTGCGAGAGTGGAACAA 100
Mutated
TTGCGAGAGTGGAACAA GTGCGAGAGTGGAACAA 100
ATGCGAGAGTGGAACAA 88
CTGCGAGAGTGGAACAA 100
TTGCGAGAGTGGAACAA TGGCGAGAGTGGAACAA 100
TCGCGAGAGTGGAACAA 96
TAGCGAGAGTGGAACAA 100
 TTGCGAGAGTGGAACAA TTACGAGAGTGGAACAA 100
TTTCGAGAGTGGAACAA 74
TTCCGAGAGTGGAACAA 65
 TTGCGAGAGTGGAACAA TTGGGAGAGTGGAACAA 94
TTGAGAGAGTGGAACAA 44
TTGTGAGAGTGGAACAA 100
 TTGCGAGAGTGGAACAA TTGCGGGAGTGGAACAA 52
TTGCGTGAGTGGAACAA 39
TTGCGCGAGTGGAACAA 58
 TTGCGAGAGTGGAACAA TTGCGAGAGTAGAACAA 86
TTGCGAGAGTTGAACAA 40
TTGCGAGAGTCGAACAA 70
 TTGCGAGAGTGGAACAA TTGCGAGAGTGAAACAA 70
TTGCGAGAGTGTAACAA b
TTGCGAGAGTGCAACAA 10
 TTGCGAGAGTGGAACAA TTGCGAGAGTGGGACAA 56
TTGCGAGAGTGGTACAA 34
TTGCGAGAGTGGCACAA 17
 TTGCGAGAGTGGAACAA TTGCGAGAGTGGAGCAA
TTGCGAGAGTGGATCAA 8
TTGCGAGAGTGGACCAA 5
 TTGCGAGAGTGGAACAA TTGCGAGAGTGGAAGAA 6
TTGCGAGAGTGGAAAAA
TTGCGAGAGTGGAATAA
 TTGCGAGAGTGGAACAA TTGCGAGAGTGGAACGA 100
TTGCGAGAGTGGAACTA 41
TTGCGAGAGTGGAACCA 27
 TTGCGAGAGTGGAACAA TTGCGAGAGTGGAACAG 82
TTGCGAGAGTGGAACAT 89
TTGCGAGAGTGGAACAC 53
  • a Positions affected by substitutions are underlined.

  • b —, no band shift.