Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidGenotype and/or relevant characteristicsSource or reference
Strains
S. aureus
  8325-4Wild-type strain cured of all known prophages53
  RN4220Restriction minus, modification plus45
  PC6911 agrΔ::tetM(Tetr)10
  PC1839 sarA::km(Kanr)11
  PC400 sigB::Tc(Tetr)This study
  MC90 sigB+sigB::lacZ (Eryr)This study
  MC100 sigB+sigB::lacZ (Eryr)This study
  MC102 sigB+sigB::lacZ sarA::km(Eryr Kanr)This study
  PC161 sarA+sarA::lacZ (Eryr)10
  PC4030 sarA+sarA::lacZ sigB::Tc(Eryr Tetr)This study
  PC322 hla+hla::lacZ (Eryr)10
  PC4044 hla+ hla::lacZ sigB::Tc (EryrTetr)This study
  PC203 spa+spa::lacZ (Eryr)10
  PC4058 spa+ spa::lacZ sigB::Tc (EryrTetr)This study
E. coli
  DH5αφ80dlacZΔM15 recA1 endA1 gyrA96 thi-1 hsdR17(rK mK +) supE44 relA1 deoRΔ(lacZYA-argF)U169 Promega
  TP610 C600 (F thi-1 thr-1 leuB6 lacY1 tonA21 supE44 λ) hsdR hsdM recBC lop11 lig 34
Plasmids
 pAZ106LacZ transcriptional fusion vector (Ampr, E. coli; Eryr, S. aureus)41
 pUBS1 E. coli cloning vector (Ampr)25
 pDG1515Tetracycline resistance antibiotic cassette in Bluescript KS(+) (Ampr)30
 pMI1101Chloramphenicol resistance antibiotic cassette in a derivative of pBR322 (Ampr)74
 pMC42.3-kbXbaI-EcoRI PCR product containing upstream regulatory region and 5′ end of sigB cloned into pAZ106, cut with XbaI-EcoRI;sigB::lacZ (Ampr, E. coli; Eryr, S. aureus)This study
 pPC6041.1-kb EcoRI-SalI PCR product containing the entire sigB gene cloned into pUBS1 cut withEcoRI-SalI (Ampr)This study
 pPC6242.1-kb SmaI-HindIII tetracycline resistance cassette from pDG1515, end filled and cloned into pPC604 cut with EcoRV (Ampr)This study
 pPC7161.5-kb BamHI chloramphenicol resistance cassette from pMI1101 cloned into pPC624 cut with BamHI (Ampr, E. coli; Eryr, S. aureus)This study