Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidDescriptionSource or reference
E. coli
 DH5αMCRFmcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 phoA supE44 λ thi-1 gyrA96 relA1BRL
 S17-1Pro (rm+) contains integrated plasmid RP4-2-Tc::Mu-Km::Tn7, TprSmr42
S. putrefaciens
 MR-1Lake Oneida isolate28
 MR-1RSpontaneous mutant of MR-1, RifrThis work
 SR-21MR-1R butfreC::Tn5, RifrKmrThis work
 SRC-21SR-21 carrying pSC52, Rifr Kmr TcrThis work
Plasmids
 pUC119Cloning and sequencing vector, AmprBRL
 pSPORT1Cloning and sequencing vector, AmprBRL
 pRK415IncP plamid, Tcr15
 pSUP1011pACYC184 derivative carrying RP4 oriT and Tn5, CmrKmr42
 pSRF212pSPORT1 withfreB-containing 6.2-kb EcoRI-BamHI fragmentThis work
 pAB52pUC119 withfreBC-containig 5.8-kbEcoRI-HindIII fragmentThis work
 pSC52pRK415 with freBC-containing 5.8-kbEcoRI-HindIII fragmentThis work