Table 3.

Mutational analysis of transcriptional activation at thetpl promoter

StrainRelevant genotypeβ-Galactosidase activitya
GlucoseGlycerol
SP1312 (Ptpl-lacZ+)mut1tyrR+, G→A in box A57.9555
SP1312 (Ptpl-lacZ+)mut2tyrR+, G→A in box B111.0549
SP1312 (Ptpl-lacZ+)mut3tyrR+, G→A and C→T in box C47.885.3
SP1313 (Ptpl-lacZ+)mut3Δ(tyrR), G→A and C→T in box C22.022.6
SP1312 (Ptpl-lacZ+)Δ64tyrR+, 64-nucleotide deletion41.2278
SP1313 (Ptpl-lacZ+)Δ64Δ(tyrR), 64-nucleotide deletion13.130.0
SP1312 (Ptpl-lacZ+)Δ130tyrR+, 130-nucleotide deletion34.188.9
SP1313 (Ptpl-lacZ+)Δ130Δ(tyrR), 130-nucleotide deletion17.155.2
SP1312 (Ptpl-lacZ+)mut2,3tyrR+, G→A and C→T in box C, G→A in box B42.072.7
SP1313 (Ptpl-lacZ+)mut2,3Δ(tyrR), G→A and C→T in box C, G→A in box B28.338.2
SP1312 (Ptpl-lacZ+)Δ64CtyrR+, 64-nucleotide deletion, box C is replaced by box A93.6170
SP1313 (Ptpl-lacZ+)Δ64CΔ(tyrR), 64-nucleotide deletion, box C is replaced by box A12.128.7
SP1312 (Ptpl-lacZ+)Δ179CtyrR+, 179-nucleotide deletion, box C is replaced by box A65.972.9
SP1313 (Ptpl-lacZ+)Δ179CΔ(tyrR), 179-nucleotide deletion, box C is replaced by box A14.322.0
SP1313 (Ptpl-lacZ+)Δ64C/pJC100Δ(tyrR) ptyrR+ Δ64C316527
SP1313 (Ptpl-lacZ+)Δ179C/pJC100Δ(tyrR) ptyrR+ Δ179C410ND
SP1313 (Ptpl-lacZ+)Δ179C/pJC100/pJC136Δ(tyrR) (ptyrR+) Δ179C614ND
  • a β-Galactosidase activity is expressed in Miller units. Cultures were grown in glucose or glycerol as indicated. ND, not determined.