Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidGenotype or relevant characteristicsaOrigin (reference)
S. aureus
 RN4220Restriction negative, modification positiveR. Novick 40
 8325-4Wild-type strain cured of known prophagesR. Novick48
 PC6911 agrΔ::tetMTcr Laboratory stock 12, 51
 PC2429 sarA+sarA::km KmrEryr This study
 PC1839 sarA::kmKmr This study
 PC18391 sarA::km agrΔ::tetM KmrTcr This study
 PC322 hla+hla::lacZ Eryr Laboratory stock 12
 PC3221 hla+hla::lacZ sarA::kmEryr Kmr This study
 PC324 hla+ hla::lacZ agrΔ::tetM EryrTcr Laboratory stock 12
 PC3241 hla+ hla::lacZ sarA::km agrΔ::tetMEryr Kmr Tcr This study
 PC203 spa+spa::lacZ Eryr Laboratory stock 12
 PC2031 spa+spa::lacZ sarA::kmEryr Kmr This study
 PC206 spa+ spa::lacZ agrΔ::tetM EryrTcr Laboratory stock 12
 PC2062 spa+ spa::lacZ sarA::km agrΔ::tetMEryr Kmr Tcr This study
 PC1072 tst::luxTcr Laboratory stock 12
 PC1091 tst::lux sarA::km TcrKmr This study
 S6Enterotoxin-producing strainS. Khan 20
 PC1700 agrΔ::tetM in S6 Tcr This study
 PC1841 sarA::km in S6 Kmr This study
 PC1845 sarA::km agrΔ::tetM in S6 TcrKmr This study
E. coliDH5αφ80dlacZΔM15 recA1 endA1 gyrA96 thi-1 hsdR17 (rK mK +)supE44 relA1 deoRΔ(lacZYA-argF)U169 Promega Corp.
Plasmids
 pUBS1 E. coli cloning vector; Apr G. Murphy 25
 pAZ106Promoterless, transcriptional lacZ fusion vector; Apr (E. coli) Eryr (S. aureus)A. Moir 38
 pDG7831.5-kb kanamycin resistance cassette in pSB118; Apr P. Stragier29
 pPC9051.3-kbSalI-BamHI cut PCR fragment containing the complete sar operon in SalI-BamHI-cut pUBS1; Apr This study
 pH41.5-kbEcoRI fragment from pDG783 containing the kanamycin resistance cassette in EcoRI-cut pPC905; Apr This study
 pH4A53.3-kb PvuII fragment containing sarA::km inSmaI-cut pAZ106; Apr (E. coli) Eryr (S. aureus)This study
  • a Abbreviations: Tcr, Kmr, Eryr, and Apr, resistance to tetracycline, kanamycin/neomycin, erythromycin/lincomycin, and ampicillin, respectively.