Table 1.

Purification of M. thermoautotrophicum SerRS

FractionTotal amt of protein (mg)Sp act (pmol min−1 mg−1)Total activity (pmol)Enrichment factor (fold)Yield (%)
S-100307.47.62,334
Q-Sepharose44.143.31,9055.781.6
Phenyl-Sepharose4.6210950414.321.6
MonoQ 5/50.614172545510.9
Superose120.0166,5301058594.5