Table 1.

Strains, plasmids, and phages used in this study

Strain, plasmid, or phageRelevant genotypeSource or reference
E. coli strains
 DH5αΔ(argF-lac)U169(φ80ΔlacZM15) recA 30
 JRG1999MC1000 ΔcrpT8S. T. Cole, Paris, France
 JRG1728MC1000 Δ(tyrR-fnr-rac-trg)17 zdd-230::Tn9 39
 JRG3834MC4100 [λ RS45:: dcuA′-lacZYA]This work
 JRG3835MC4100 [λRS45:: dcuB′-lacZYA]This work
 JRG3836MC1000 [λRS45:: dcuA′-lacZYA]This work
 JRG3837MC1000 [λRS45:: dcuB′-lacZYA]This work
 JRG3838JRG3834fnr This work
 JRG3839JRG3835fnr This work
 JRG3840JRG3834arc This work
 JRG3841JRG3835arc This work
 JRG3842JRG3835narL This work
 JRG3843JRG3835narP This work
 JRG3845JRG1999 [λRS45:: dcuA′-lacZYA]This work
 JRG3846JRG1999 [λRS45:: dcuB′-lacZYA]This work
 MC1000ΔlacX74Δ( araABC-leu) 33
 MC4100Δ(argF-lac)U169rpsL 33
 RKP3580narL215::Tn10R. K. Poole, Sheffield, United Kingdom
 RKP3655narP258::Tn10dR. K. Poole, Sheffield, United Kingdom
 RM315MC4100 ΔarcA1zjj ::Tn10 31
Plasmids
 pGS73 aspA dcuA 13
 pGS78 dcuB fumB 12
 pGS744pBR322 + 4.7-kbBamHI-EcoRI dcuB-fumB fragmentThis work
 pGS745pBR322 + 6.2-kb SphI-SalIaspA-dcuA fragmentThis work
 pGS748pUC118 + 1.3-kb dcuA fragmentThis work
 pGS749pUC119 + 1.3-kb dcuB fragmentThis work
 pGS929pRS415 +dcuA′-lacZYA This work
 pGS931pRS528 +dcuB′-lacZYA This work
 pRS415/528 lacZYAtranscriptional fusion vectors 35
Phages
 P1vir1 26
 λRS45 35