Table 2.

Characterization of minor SASP in B. subtilis sporesa

Protein bandbN-terminal sequenceGene identified in B. subtilisgenomecNo. of amino acid residuesdNew gene designation
1  SENRHENEENRRDA- 3353125 to 335326847 sspG
2  TKNQNQYQQPN- tlp e 82 tlp
3  MNIQRAKEIVES- yfjU 59 sspH
4  ASRNKLVVPGVE- andMDLNLRHAVIAN- sspD 63 sspD
ysfA 71sspI
5  AQQSRSRSNNNN- f sspC 71 sspC
6  GFFNKDKGKRSE- 3420667 to 342053045 sspJ
7  VRNKEKGFPYEN- 927771 to 92762249 sspK
8  VKRKANHVINGM- cotK 47 cotK
9  MKKKDKGRLTGG- 2310101 to 231022642 sspL
10 MKTRPKKAGQQK- 2338912 to 233901334 sspM
  • a Protein bands were generated and sequenced as described in Materials and Methods.

  • b As labeled in Fig. 1, lane e.

  • c Gene names are as in the B. subtilis genomic sequence available on the World Wide Web atwww.pasteur.fr/Bio/SubtiList.html. Numbers given are the locations in the genome of regions coding for proteins not previously identified as ORFs. The first and second numbers are those of the first nucleotide of the start codon and the last nucleotide of the coding sequence, respectively.

  • d Residues in the intact protein; the N-terminal methionine of a number of the proteins is removed posttranslationally.

  • e This was the major sequence observed; however, there were also significant amounts of the same sequence but with an N-terminal methionine (∼20% of the total) or beginning at the first lysine residue (∼30% of the total).

  • f Sequence from reference46.