Table 2.

Expression of different transcriptional fusions inL. casei strains

StrainRelevant genotypeSugarβ-Glucuronidase activitya (nmol/min/mg [dry wt])
pNZlacpNZRAT
BL23Lactose101.3 ± 7.22,318.5 ± 443.5
Ribose139.7 ± 15.61,025.0 ± 17.5
Glucose6.3 ± 1.815.2 ± 2.3
BL153 lacE Ribose144.4 ± 7.92,964.0 ± 262.0
Glucose7.3 ± 1.910.5 ± 0.6
BL154 lacT Ribose94.7 ± 4.947.6 ± 13.4
Glucose9.5 ± 0.85.5 ± 2.2
BL155 lacF Ribose120.3 ± 7.52,860.0 ± 545.0
Glucose15.1 ± 5.48.0 ± 2.4
  • a Measured with p -nitrophenyl-β-d-glucuronic acid in permeabilized cells grown with 0.5% sugar to an optical density at 550 nm of 0.8. The values (means and standard deviations) are from three independent experiments.