Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristicsReference or source
Bacterial strains
E. chrysanthemi 3937 derivatives
  A350 lacZ2 5
  A1077 lacZ2 kdgR::CmLaboratory collection
  A1671 lacZ2 pelW::MudI1734 Km 5
  A3439 lacZ2 kdgR::Cm pelW::MudI1734 KmThis work
Escherichia coli
  NM522Δ(lac-proAB) Δ(mcrB-hsdSM)5 supE thi (F′ proAB lacI q lacZΔM15)Laboratory collection
  BL21(DE3) E. coli B; F dcm ompT hsdS gal λ(DE3); T7 polymerase gene under the control of the lacUV5 promoterStratagene
  K-38HfrC(λ)phoA4 pit-10 tonA22 ompF627 relA1 Laboratory collection
Plasmids
 pBS AppBluescript SK(+); Apr Stratagene
 pET-20b(+)Carries the N-terminal pelB signal sequence; Apr Novagen
 pGP1.2The T7 RNA polymerase gene under the control of the λPL promoter and the cI857 repressor; Kmr 38
 pT7-6T7Φ10; Apr 38
 pNV9pBR325 derivative carrying the 3.2-kb EcoRV-EcoRI fragment with pelW 5
 pBS-KCpBS derivative with the 2.9-kbEcoRV-ClaI fragment from pNV9;pelW + This work
 pT7-KCpT7-6 derivative with the 3-kb EcoRV-EcoRI fragment from pNV9; pelW + This work
 pT7-KCsppET-20b(+) derivative with the 2.5-kbHpaI-HindIII ′pelW fragment under the control of the pelB signal sequenceThis work
 pOGL-10pBS derivative carryingogl 28
 pT7-OGLpT7-5 derivative with the 1.6-kb PinAI-PstI fragment from pOGL-10; ogl + This work
 pTaXpT7-5 derivative with pelX; Apr 34
 pT7-pelD pT7-5 derivative with pelD; Apr 39