Table 1.

Chemotactic response of V. anguillarum to amino acids and carbohydrates identified and quantified in rainbow trout intestinal mucusa

Amino acid or carbohydrateRelative responsebConcentration (mM) in intestinal mucusc
Amino acids
l-Alanine<32.53
l-Asparagine<30.73
l-Aspartic acid<31.05
l-Glutamic acid63.03
l-Glutamine492.02
 Glycine243.16
l-Histidine450.97
l-Isoleucine421.09
l-Leucine72.0
l-Lysine<31.74
l-Phenylalanine<30.99
l-Proline<31.51
l-Serine371.57
l-Threonine511.33
l-Tryptophan<30.2
l-Tyrosine<31.67
l-Valine<31.69
Carbohydrates
l-Fucose60.20d
d-Galactose<30.17d
d-Glucose120.07
myo-Inositol<30.12
d-Mannose140.05
  N -acetyl-d-galactosamine<30.08
N-acetyl-d-glucosamine<30.11
d-Ribose<30.46
 2-Deoxy-d-ribose<30.26
d-Xylose90.13
  • a The chemotactic response of wild-typeV. anguillarum NB10 to individual mucus-associated amino acids and carbohydrates at 0.1, 1.0, and 10 mM concentrations was measured in a capillary assay and is expressed in terms of a relative response (the ratio of bacterial accumulation in substrate-containing capillaries to that in control buffer-containing capillaries).

  • b Relative response for each substrate when present at a concentration of 1.0 mM is illustrated.

  • c The concentration of the individual components in undiluted crude intestinal mucus gel.

  • d Concentration was determined following hydrolysis of the sample.