Table 1.

Plasmids used in this studya

PlasmidRelevant characteristicsSource or reference
Vectors
 pACT2.2GAL4AD plasmid 2
 pAS2GAL4BD plasmid 2
 pSW172Tetrbroad-host-range IncP cloning vector 12
 pXZ151Kanr pSW172 derivativeThis study
GAL4 expression constructs
 pXZaE1pACT2.2 with virE1 geneThis study
 pXZaE2pACT2.2 with virE2 geneThis study
 pXZbE1pAS2 with virE1 geneThis study
 pXZbE2pAS2 with virE2 geneThis study
 pSE1111pACT2.2 with SNF4 2
 pSE1112pAS2 with SNF1 2
 pXZbE201 to pXZbE212pAS2 with virE2 truncations fused to GAL4BD This study
 pXZaE201 to pXZaE212pACT2.2 with virE2 truncations fused toGAL4AD This study
 pXZ741 to pXZ750pACT2.2 withvirE2::i31 alleles fused toGAL4AD This study
 pXZ751 to pXZ760pAS2 withvirE2::i31 alleles fused toGAL4BD This study
virE1 andvirE2 expression constructs
 pPC725pBCSK+.NdeI withvirE2; NdeI site at 5′ endThis study
 pXZ46pBSIISK+.NdeI withvirE2 expressed from Plac This study
 pXZ27pBSIIKS+ with virE2expressed from PvirB This study
 pPC731pBSIIKS+.NdeI withvirE2 expressed from PvirE This study
 pXZ425pSW172 with virE2 expressed fromPvirB This study
 pXZ237pBSIIKS+.NdeI withvirE1 and virE2 expressed fromPlac This study
 pPC732pBSIIKS+.NdeI withvirE1 and virE2 coexpressed fromPvirE This study
 pXZ235pPC731 withvirE1 containing a translational stop signal at codon 6 andvirE2 expressed from PvirE This study
 pXZ236pXZ237 with virE1 containing a translational stop signal at codon 6 and virE2 expressed from Plac This study
virE2 insertion and deletion plasmids
 pXZ201 to pXZ212pBSIIKS+ with virE2truncations expressed from PvirB This study
 pXZ721 to pXZ730pBSIIKS+.NdeI withvirE2::i31 alleles expressed fromPlac This study
 pXZ761 to pXZ770pBSIIKS+ withvirE2::i31 alleles expressed fromPvirB This study
 pXZ731pBSIISK+.NdeI with an out-of-frame i31 insertion at codon 11 of virE2 This study
 pXZ738pBSIISK+.NdeI with an out-of-frame i31 insertion at codon 468 of virE2 This study
 pXZ427pXZ425/pXZ761 cointegrate with virE2and virE2.39::i31 expressed from independent PvirB promotersThis study
Hybrid protein constructs
 pXZ234pBSIIKS+ with his-virE2expressed from Plac This study
 pXZ238pBSIIKS+ withGAL4AD::virE2 expressed fromPvirB This study
 pCSK100pBCSK+ with gfp coding for M2 mutant expressed from Plac 36
 pXZ428pED32 IncP plasmid withgfp::virE2 expressed fromPvirB This study
 pXZ66pBKSK+.NdeI withvirE2::gfp expressed fromPlac This study
 pXZ479pBSIIKS+ withvirE2::gfp expressed fromPvirB This study
 pBG3pBSIIKS+ withvirE2::phoA expressed fromPvirB This study
 pXZ426pXZ425/pXZ66 cointegrate with virE2 andvirE2::gfp expressed from independentPvirB promotersThis study
 pXZ168pBSIISK+.NdeI withvirE1::gfp expressed fromPlac This study
 pXZB169pXZ425/pXZ168 cointegrate with virE2 andvirE1::gfp expressed from independentPvirB promotersThis study
  • a Except where indicated, ColE1 plasmids expressing virE constructs were ligated to IncP plasmid pSW172 or pXZ151 for introduction into A. tumefaciens; the cointegrate plasmid in A. tumefaciens is given the ColE1 plasmid name plus a B (for broad host range).