Table 2.

Photopigment synthesis and photosynthetic gene expression in reg mutants of Rhodobacter capsulatus complemented with reg genes from various species

StrainBChl content (nmol/mg)cExpression ofpuf operond
−O2+O2−O2+O2
Wild type (R. capsulatus)34.50.5544 ± 3015 ± 5
TB2(pJRD215)a vector only13.90.340 ± 109 ± 5
TB2(pMWS3.1) +Rhodobacter regA-senC 37.20.7382 ± 5062 ± 20
TB2(pMCS003) +Rhodovulum regA-senC 51.50.61,971 ± 150125 ± 25
TB2(pMCR001) +Roseobacter regA-senC 42.50.6315 ± 3060 ± 15
SD01(pJRD215)b vector only21.60.5135 ± 7018 ± 10
SD01(pCSM9e) +Rhodobacter regB-senC 42.80.51,050 ± 145108 ± 40
SD01(pMCS010) +Rhodovulum regB 45.70.5855 ± 15060 ± 20
SD01(pMCR010) +Roseobacter regB-senC 40.10.4499 ± 12563 ± 25
  • a TB2; R. capsulatusregA).

  • b SD01; R. capsulatusregB).

  • c BChl content in membranes (nanomoles per milligram of membrane protein). Data are based on the average of three independent assays. Uncertainty limits in this assay are within 5% in all transconjugants and the wild type. −O2, anaerobic photosynthetic growth; +O2, aerobic-dark growth.

  • d Values are β-galactosidase activity (micromoles of o-nitrophenol-β-d-galactoside hydrolyzed per minute per milligram of protein) of strains with thepuf::lacZ translational fusion in pCB532Ω (4).