Table 1.

Bacteria and plasmids used in this study

Strain or plasmidCharacteristic(s)Source or reference
E. coli
 DH5α supE44 ΔlacU169 (φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1λ Bethesda Research Laboratories
 JM109 endA1 recA1 gyrA96 hsdR17 supE44λ Δ(lac-proAB)/F′ [traD36proAB+ lacZΔM15] 68
 JM109 λ pir JM109 lysogenized with λpir bacteriophage 47
 S17-1Tpr Smr hsdR pro recARP4-2-Tc::Mu-Km::Tn7 in chromosome 61
 S17-1 λpir S17-1 lysogenized with λ pirbacteriophage 15
 XL1-Blue MR. recA1 endA1 gyr-96 thi-1 hsdR17 supE44 relA1 lac/F′ [proAB+ lacZΔM15::Tn10(Tcr)] 11
 C600/pDPT51Mobilizing vector 63
R. capsulatus
 ATCC 11166Wild type 49
 TB2SB1003regAΔEcoRI-PflMI::Kmr 7
 SD01SB1003 regBΔ640bp N-terminus::Kmr 18
R. sulfidophilum
 W4Wild type 27
 RESA1 regAΔPinAI-PinAI::Kmr This study
 RESB20 regB::Kmr This study
R. denitrificans
 OCh114Wild type 57
Plasmids
 SuperCos 1Apr; cosmid vectorStratagene
 pUC118Apr; cloning vector 68
 pJP5603Kmr; R6K-based suicide vector 47
 pSM3065Tcr; pJP5603 derivativeThis study
 pUC7TcApr Tcr; source of the Tcr gene 33
 pUCKM1Apr Kmr; source of the Kmr gene 53
 pCB532ΩApr Smr Spr; translational fusion of pufQ tolacZ 4
 pJRD215Kmr Smr; broad-host-range cosmid vector 14
 pSLAApr; 5.0-kb EcoRI fragment (R. sulfidophilum regB-senC-regA) in pUC118This study
 pROAApr; 5.5-kb EcoRI fragment (R. denitrificans regB-senC-regA) in pUC118This study
 pSLKm4Apr Kmr; 6.3-kb fragment (R. sulfidophilum regAΔPinAI-PinAI::Kmr) in pUC118This study
 pSA10Apr KmrTcr; 9.4-kb fragment (pSLKm4 digested with KpnI) in pSM3065This study
 pSB07Kmr; 0.4-kbSacI-SalI fragment (part of R. sulfidophilum regB) in pJP5603This study
 pMWS3.1Kmr Smr; 12-kb fragment (R. capsulatus senC-regA) in pJRD215 55
 pCSM9eKmrSmr; 5.1-kb fragment (R. capsulatus regB-senC) in pJRD215 42
 pMCS003KmrSmr; 2.8-kb EcoRI-NruI fragment (R. sulfidophilum senC-regA) in pJRD215This study
 pMCS010Kmr Smr; 2.6-kbEcoRI-NdeI fragment (R. sulfidophilum regB) in pJRD215This study
 pMCR001KmrSmr; 3.2-kb EcoRI-SacI fragment (R. denitrificans senC-regA) in pJRD215This study
 pMCR010Kmr Smr; 2.5-kbHincII fragment (R. denitrificans regB-senC) in pJRD215This study