Table 2.

Chemotactic response of V. anguillarum to lipids identified and quantified in rainbow trout intestinal mucusa

Lipid groupdRelative responsebConcentration (μg/ml) in intestinal mucusc
Free FAs (16:0, 18:0, 18:1, 20:1, 20:4, 20:5, 22:6)<3500
Mono- and diglycerides (MPG, MSG, DSG)<3200
Cholesterol<3150
Phospholipids (PC, PI)<3100
Bile acids
 TC516
 TCDOC54
  • a The chemotactic response of wild-typeV. anguillarum NB10 to individual mucus-associated lipids at 0.1 and 1.0 mM concentrations was measured in a capillary assay and expressed in terms of a relative response (the ratio of bacterial accumulation in substrate-containing capillaries to that in control buffer-containing capillaries).

  • b Relative response for each substrate when present at a concentration of 1.0 mM is illustrated.

  • c The concentration of the individual components in undiluted intestinal mucus gel derived from their respective measured concentrations in a chloroform-methanol extract of the 1/10-diluted intestinal mucus homogenate.

  • d MPG, monopalmitoylglycerol; MSG, monostearoylglycerol; DSG, distearoylglycerol; PC, phosphatidylcholine; PI, phosphatidylinositol.