Table 1.

Bacterial strains, bacteriophages, and plasmids used throughout this study

Strain, phage, or plasmidGenotype or relevant propertiesSource or reference
Strains
S. aureus 187Host for phage 187; assay for lytic activityH. W. Ackermann
M. luteus WS2331Assay for lytic activityLaboratory stock
E. coli
  JM109recA1 endA1 gyrA96 thi hsdR17 supE44 relA1Δ(lac-proAB) [F′ traD36 proAB lacIqZΔM15]Laboratory stock
  JM109(DE3)::DE3, T7 RNA polymerase underlacUV5 controlPromega
  LE392FhsdR514 supE44 supF58 lacY1 galK2 galT22 metB1 trpR55 (phage host; permissive for λgt11)Promega
  HB101FhsdS20 supE44 recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 (phage host; nonpermissive for λgt11)Promega
  W3110F λATCC 27325
Phages
 187Wild type, B1 siphovirus; approx. 45-kbp double-stranded DNA genomeH. W. Ackermann
 λWild typeATCC 23724-B2
 λgt11cIts857; Sam100; lacpromoter for expression of cloned genesPromega
 λgt11::hol187hol187inserted into EcoRI site of λgt11This work
 λgt11::hol187-SModifiedhol187 (encoding Hol187 ΔM1L2) inserted intoEcoRI site of λgt11This work
 λgt11::hol187-LModifiedhol187 (encoding Hol187 M3L) inserted into EcoRI site of λgt11This work
 λgt11::hol187-ChModifiedhol187 (encoding Hol187 V7D G8D) inserted intoEcoRI site of λgt11This work
Plasmids
 pSP722.4-kb cloning and expression vector; T7 promoter; AmprPromega
 pBluescript II (SK−)3.0-kb cloning and expression vector; lac promoter; AmprStratagene
 pPL1871,112-bp AluI fragment from phage 187 ligated into SmaI site of pSP72This work
 pPL187-F1471-bp PCR product (N-terminal domain of Ply187) inserted into BamHI site of pSP72This work
 pPL187-F2891-bp PCR product (C-terminally truncated Ply187) inserted into BamHI site of pSP72This work
 pPL187-F31,887-bp PCR product (complete Ply187) inserted intoBamHI site of pSP72This work
 pPL187-F4421-bp PCR product (central portion of Ply187) inserted into BamHI site of pSP72This work
 pPL187-F51,416-bp PCR product (N-terminally truncated Ply187) inserted into BamHI site of pSP72This work
 pHOL187188-bp PCR product (encoding Hol187) (Fig. 1) inserted into EcoRI site of pBluescriptThis work
 pHOL187-S182-bp PCR product (encoding Hol187 ΔM1L2) inserted into EcoRI site of pBluescriptThis work
 pHOL187-L188-bp PCR product (encoding Hol187 M3L) inserted into EcoRI site of pBluescriptThis work
 pHOL187-Ch188-bp PCR product (encoding Hol187 V7D G8D) inserted into EcoRI site of pBluescriptThis work