Table 1.

B. subtilis strains and plasmids used

Strain or plasmidRelevant genotypeReference, source, or construction
Bascillusstrains
 SJV6 Pspac ::obg A. Grossman
 PY22 trpC2 P. Youngman
 AG514 spo0A::cat A. Grossman
 BSA46 trpC2 Spβ ctc::lacZ 3
 XS15 trpC2 rsbX::spc rsbU194VA Spβ ctc::lacZ 30
 BSA400 trpC2 rsbT::pHV501T(Pspac ::rsbT) pHV501T→PY22
 BSA419 trpC2 Pspac ::rsbT Spβ ctc::lacZ BSA46 →BSA400
 BSJ-5 trpC2 Pspac ::obg SJV6→PY22
 BSJ-6 trpC2 Pspac ::obg Spβ ctc::lacZ BSA46→BSJ-5
 BSJ-9 trpC2 Pspac ::obg (pJM2) Spβ ctc::lacZ pJM2→BSA46
 BSJ-10 trpC2 P spac::obg rsbX::spc rsbU194VA Spβ ctc::lacZ XS15→BSJ-6
 BSJ-11 trpC2 Pxyl ::obg pJM4→PY22
 BSJ-12 trpC2 Pxyl ::obg Spβ ctc::lacZ BSA46→BSJ-11
 BSJ-13 trpC2 Pxyl ::obg Pspac ::rsbT Spβ ctc::lacZ BSJ-11→BSA419
 BSJ-16 trpC2 spo0A::cat AG514→PY22
 BSJ-17 trpC2 spo0A::cat Spβ ctc::lacZ BSA46→BSJ-16
Plasmids
 pUS19 AprSpecr 4
 pUSX19Apr Specr Pxyl xylR This study
 pACT-2Apr vector with AD of GAL4Clontech
 pGAD-10Apr vector with AD of GAL4Clontech
 pGAD-GLApr vector with AD of GAL4Clontech
 pX-2Apr PxylxylR 20
 pUV31Apr protein fusion of RsbX with BD of GAL436
 pUV76Apr protein fusion of RsbT with BD of GAL436
 pUV145Apr protein fusion of RsbU with BD of GAL436
 pJM2AprKanr Pspac ::obg This study
 pJM4Apr Specr Pxyl ::obg 150 xylR This study
 pDG148Apr Kanr Pspac lacI P. Stragier
 pHV501Apr Ermr Pspac lacI lacZ S. D. Ehrlich
 pHV501TApr Ermr Pspac ::rsbT lacI This study
 pDT11Apr Kanr Pspac ::rsbT lacI 30
 pBluescriptApr 26