Table 3.

Complementation analysis of mini-Tn5Ptrb mutants of pRKtrb

pRKtrb::mini-Tn5PtrbmutantGene mutatedpTiC58ΔaccR:: Tn3HoHo1 mutantaTransfer frequency of pTiC58ΔaccR:: Tn3HoHo1 derivative
NoneNonepDEK-11b 9.0 × 10−2
pRKtrb-14 traI pDEK-73.1 × 10−2
pRKtrb-4 trbB pDEK-72.3 × 10−1
pRKtrb-13 trbB pDEK-72.6 × 10−1
pRKtrbΔCc trbC pDEK-72.2 × 10−3
pRKtrb-11 trbD pDEK-78.8 × 10−2
pRKtrb-2 trbE pDEK-215<10−7
pRKtrb-5 trbE pDEK-2154.1 × 10−3
pRKtrb-15 trbE pDEK-2151.6 × 10−4
pRKtrb-16 trbJ pDEK-2159.4 × 10−5
pRKtrb-1 trbL pDEK-2171.4 × 10−3
pRKtrb-8 trbF pDEK-2174.1 × 10−4
pRKtrb-12 trbF pDEK-2175.1 × 10−4
pRKtrb-3 trbG pDEK-93.9 × 10−3
pRKtrb-7 trbH pDEK-94.4 × 10−3
  • a All derivatives of pTiC58ΔaccR::Tn3HoHo1 except pDEK-11 failed to transfer at a detectable frequency in the absence of the complementing plasmids.

  • b pDEK-11 is a derivative of pTiC58ΔaccR with a Tn3HoHo1 insertion innocR (42).

  • c Contains a replacement mutation intrbC as described in the text.