Table 4.

Conjugal transfer frequency of pTiC58ΔaccR::mini-Tn5Ptrbmutants and complementation of these mutants with trbgene clones

Mutant Ti plasmidtrb gene mutatedTransfer frequency when mated from:
NT1 (Ti plasmid only)UIA5
Ti plasmid only+pKK38ASH+trb ORF clone
pTra17-52None1.6 × 10−1 2.4 × 10−2 NTa NT
pPLK-4 trbB 7.6 × 10−4 <10−8 <10−8 8.3 × 10−5
pPLKΔC trbC 6.4 × 10−4 <10−8 <10−8 4.4 × 10−2
pPLK-11 trbD 1.2 × 10−5 <10−8 <10−8 5.6 × 10−3
pPLK-5 trbE 2.1 × 10−6 <10−8 <10−8 2.0 × 10−3
pPLK-16 trbJ <10−7 <10−8 <10−8 3.8 × 10−6
pPLKΔK trbK 5.5 × 10−2 2.9 × 10−3 1.2 × 10−4 1.2 × 10−3
pPLK-1 trbL 3.3 × 10−6 <10−8 <10−8 2.0 × 10−3
pPLK-12 trbF 6.6 × 10−4 <10−8 <10−8 1.8 × 10−2
pPLK-3 trbG 4.7 × 10−6 <10−8 <10−8 1.1 × 10−4
pPLK-7 trbH 3.7 × 10−4 <10−8 <10−8 2.0 × 10−4
pPLK-17 trbI 8.7 × 10−5 5.0 × 10−6 2.8 × 10−6 4.1 × 10−4
  • a NT, not tested.