Table 1.

Bacterial strains and plasmids

Strain or plasmidRelevant genotype or phenotypeReference or construction
E. coli K-12 strains
 BD1302ΔoxyR; autoaggregating 13
 CC118λpir λpir integrated on the chromosome 19
 HEHA3BD1302 Δfim::kan This study
 HEHA10BD1302 flu::tet This study
 MS7PC31 Δfim::kan 37
 PC31 fim + 23
Plasmids
 pACYC184CamrTetr 10
 pBR322AmprTetr 7
 pGP704Ampr; R6K-based origin (pir) 19
 pHHA130 oxyR + A 2,040-bp PCR fragment (primers 5 and 6) containing the oxyR gene from PC31 inserted into the HindIII site of pACYC184
 pHHA145 StyI site deletedpBR322 cut withStyI, made blunt with Klenow polymerase, and religated
 pHHA146 flu + A 3,550-bp PCR fragment containing the flu gene from PC31 inserted into theEcoRI/BamHI site of pBR322
 pHHA154 flu + A 3,550-bp PCR fragment containing the flu gene from PC31 inserted into theEcoRI/BamHI site of pHHA145
 pHHA159 flu::tet A 1,724-bpBsaAI/SspI fragment containing the tetgene inserted into the (blunted) StyI site of pHHA154
 pHHA165 flu::tetA 5,450-bpEcoRI fragment containing theflu::tet construct inserted into theEcoRI site of pGP704
 pLBJ311Δfim::kan 37
 pLIH14Has all fim genes exceptfimA 25
 pMAS32 lacUV5::fimACDFGH Part of the fim gene cluster (fimA to fimH) inserted behind the isopropyl-β-d-thiogalactopyranoside-induciblelacUV5 promoter
 pPAP5Contains the papgene cluster 27
 pPKL4Contains thefim gene cluster 23
 pPKL5Contains the fimBEAICDgenes 21
 pPKL9 fimB + 20
 pPKL143Contains the foc gene cluster 22