Table 2.

Mini-Tn5Ptrb restores TraR-AAI-dependent lacZ expression of a promoterlesstrbE::lacZ reporter fusion

Test strainaβ-Galactosidase activity (U/109 CFU)
−TraR+TraRb
−AAI+AAIc−AAI+AAI
pPLE2-2534146NTd
pHM256756
pHM25-7055485
  • a All plasmids are harbored in NT1.

  • b TraR was supplied by thetraR-expressing clone pSVB33.

  • c Synthetic AAI was added to the culture at a concentration of 25 nM.

  • d NT, not tested. The strain itself produces AAI.