Table 3.

Expression of Φ(invF-lacZYA), Φ(sigD-lacZYA), and Φ(sicA-lacZYA) in E. coli a

Reporter genotype (name)β-Galactosidase activityb (U)
hilA+invF+hilA+invF+
Φ(invF-lacZYA) (pHD3)c 2,798NDND
Φ(sigD-lacZYA) (pHH21)d 848981
Φ(sicA-lacZYA) (pHD11)d <1<1<1
  • a See Table 1 for descriptions of plasmids.

  • b Average of duplicate β-galactosidase assays performed per culture, representative of several assays done on different days. The standard deviation of β-galactosidase activity was less than 10% between assays. ND, not determined.

  • c The control strain, DH5α containing pHD3 and the cloning vector pWSK130, produced 2 U of β-galactosidase activity. hilA was provided by philA.

  • d The amount of β-galactosidase activity produced by strains containing the cloning vector pWSK130 was comparable to the values obtained with hilA provided intrans. Due to plasmid incompatibility constraints, differenthilA and invF constructs were used. For the pHH21 assays, hilA was provided by philA andinvF was provided by pHD17 (Table 1). For the pHD11 assays,hilA was provided by pVV214 and invF was provided by pHD9-1.