Table 4.

Complementation of the ΔinvF mutant for invasion of HEp-2 cells

StrainMean % invasion ± SDa (% of wild-type level)
No plasmidpWKS130 (vector)pHD9 (invF+)philA
SL134412.4 ± 0.3 (100)7.7 ± 1.4 (100)7.1 ± 1.3 (100)8.0 ± 2.0 (100)
SVM579 (ΔinvF)0.6 ± 0.1 (5)0.6 ± 0.1 (7)6.8 ± 1.3 (96)3.2 ± 0.3 (40)
SB154 (invA::Ωcat)0.02 ± 0.006 (0.1)NDNDND
SVM514 (spaS::ΩStr/Sp)0.02 ± 0.001 (0.1)NDNDND
  • a After bacteria were allowed to invade during a 1-h incubation. Values are representative of several assays done in duplicate. ND, not determined.