Table 5.

Conjugal mobilization of pPLtra by thetraI::mini-Tn5Ptrb mutant of pRKtrb can be restored by addition of AAI

Test strainAAI productionConjugal transfer frequency (transconjugants/input donor)
−AAI+AAIa
NT1(pPLtra)3.9 × 10−6 4.0 × 10−7
NT1(pPLtra, pRKtrb)+++4.7 × 10−3 NTb
NT1(pPLtra, pRKtrb-14)3.2 × 10−6 4.8 × 10−3
  • a Synthetic AAI was added at a concentration of 40 nM, and the culture was grown for an additional 6 h.

  • b NT, not tested.