Table 1.

Features of the eut locus sequence

NameaGene organizationbCodonscProteinComment
StartStopSenseOverlapGC3d.GCCAIχ2Length (aa)MWpI
′maeB−1221−7660.550.600.2920.817>151>1,6106.9One of two malic enzymes
IS200−7210IS200element; copy V
 tnpA−578−1200.460.560.2500.57215217,95810.1IS200transposase (Tnp)
 orf′1570.580.790.1921.926191,9077.5atsB-like fragment
 eutS3616960.510.540.2911.12811111,6735.7Carboxysome structural protein?
 eutP7091188+0.520.540.2430.64515917,7265.9Unknown function; ATP binding motif
 eutQ11661855+0.570.680.3981.69122924,9924.7Unknown function
 eutT18522655+0.590.680.2981.15526730,2396.4Cobalamin adenosyl transferase
 eutD265236680.620.680.3361.37333836,2667.5Resembles substrate domain of Pta
 eutM370939990.600.670.4112.002969,8436.5Carboxysome structural protein?
 eutN409943980.570.650.3152.0949910,3515.1Carboxysome structural protein?
 eutE441058130.600.690.3541.36046749,2596.9Aldehyde oxidoreductase
 eutJ58246663+0.610.720.3421.10527930,0184.9Possible chaperonin
 eutG665378280.570.710.3201.42939140,5707.2Alcohol dehydrogenase
 eutH79489195+0.570.730.3611.48937239,0535.8Membrane protein
 eutA9192105950.610.710.2970.89946749,5275.0Possible chaperonin
 eutB1060711968+0.570.730.4251.26545349,4494.7ET/NH4dsubunit
 eutC11987128830.610.700.3641.47529832,1375.9ET/NH4subunit
 eutL12893135520.600.640.3200.84121922,6964.5Carboxysome structural protein?
 eutK13565140590.600.610.2891.09216417,4217.5Carboxysome structural protein?
 PR14078Constitutive internal promoter
 eutR14107151590.540.610.2800.81535040,0557.2AraC family positive regulator
 term15677Probable terminator
 orf7915828166190.530.540.2970.38226330,0214.8Unknown
 term16673Probable terminator
 orf3316751170770.430.450.2010.78810912,0208.4Unknown
 hemF17098179970.570.650.3531.21329934,4306.4Coproporphyrinogen oxidase
18000188690.510.540.3100.55528931,65911.0N -acetylmuramylalanine amidase
  • a Component of the eut operon. The vertical line indicates the elements included in the eutoperon.

  • b The column headed “sense” gives the transcriptional direction, with right-facing arrows indicating counterclockwise on the standard Salmonella map; “overlap” indicates whether or not (+ and −, respectively) the stop codon overlaps the initiation codon of the next gene, implying potential translational coupling or interference (40a). Start and stop codon positions are in nucleotides numbered from the end of IS200.

  • c GC, fractional G+C content of the gene; 3d.GC, G+C content of codon third positions; CAI, codon adaptation index of Sharp and Li (52a); χ2, measure of codon usage bias (55a).

  • d ET/NH4, ethanolamine ammonia lyase.