Table 1.

Features of the eut locus sequence

NameaGene organizationbCodonscProteinComment
StartStopSenseOverlapGC3d.GCCAIχ2Length (aa)MWpI
′maeB −1221−7660.550.600.2920.817>151>1,6106.9One of two malic enzymes
IS200 −7210IS200element; copy V
 tnpA −578−1200.460.560.2500.57215217,95810.1IS200transposase (Tnp)
 orf′ 1570.580.790.1921.926191,9077.5 atsB-like fragment
 eutS 3616960.510.540.2911.12811111,6735.7Carboxysome structural protein?
 eutP 7091188+0.520.540.2430.64515917,7265.9Unknown function; ATP binding motif
 eutQ 11661855+0.570.680.3981.69122924,9924.7Unknown function
 eutT 18522655+0.590.680.2981.15526730,2396.4Cobalamin adenosyl transferase
 eutD 265236680.620.680.3361.37333836,2667.5Resembles substrate domain of Pta
 eutM 370939990.600.670.4112.002969,8436.5Carboxysome structural protein?
 eutN 409943980.570.650.3152.0949910,3515.1Carboxysome structural protein?
 eutE 441058130.600.690.3541.36046749,2596.9Aldehyde oxidoreductase
 eutJ 58246663+0.610.720.3421.10527930,0184.9Possible chaperonin
 eutG 665378280.570.710.3201.42939140,5707.2Alcohol dehydrogenase
 eutH 79489195+0.570.730.3611.48937239,0535.8Membrane protein
 eutA 9192105950.610.710.2970.89946749,5275.0Possible chaperonin
 eutB 1060711968+0.570.730.4251.26545349,4494.7ET/NH4 dsubunit
 eutC 11987128830.610.700.3641.47529832,1375.9ET/NH4subunit
 eutL 12893135520.600.640.3200.84121922,6964.5Carboxysome structural protein?
 eutK 13565140590.600.610.2891.09216417,4217.5Carboxysome structural protein?
 PR 14078Constitutive internal promoter
 eutR 14107151590.540.610.2800.81535040,0557.2AraC family positive regulator
 term 15677Probable terminator
 orf79 15828166190.530.540.2970.38226330,0214.8Unknown
 term 16673Probable terminator
 orf33 16751170770.430.450.2010.78810912,0208.4Unknown
 hemF 17098179970.570.650.3531.21329934,4306.4Coproporphyrinogen oxidase
18000188690.510.540.3100.55528931,65911.0N -acetylmuramylalanine amidase
  • a Component of the eut operon. The vertical line indicates the elements included in the eutoperon.

  • b The column headed “sense” gives the transcriptional direction, with right-facing arrows indicating counterclockwise on the standard Salmonella map; “overlap” indicates whether or not (+ and −, respectively) the stop codon overlaps the initiation codon of the next gene, implying potential translational coupling or interference (40a). Start and stop codon positions are in nucleotides numbered from the end of IS200.

  • c GC, fractional G+C content of the gene; 3d.GC, G+C content of codon third positions; CAI, codon adaptation index of Sharp and Li (52a); χ2, measure of codon usage bias (55a).

  • d ET/NH4, ethanolamine ammonia lyase.