Table 7.

Aerobic phenotypes of eut deletions

ClassaDeletionRemovedStrainGrowthb
−Tc+Tc
1eut+(Tcr)TT134403
Del1955All eutTT206060
Δeut-300eutJ–eutGTT191873
Δeut-301eutG–eutHTT191883
Δeut-302eutD–eutMTT191893
Δeut0333eutP–eutDTT205823
2eut+(Tcr)TT1344033
Δeut-338 (T-POP)ΔeutE–eutKTT205850c0
Δeut–335(T-POP)ΔeutA–eutKTT2058200
Δeut-336(T-POP)eutS–eutKTT2058300
Δeut-337(T-POP)eutJ–eutKTT2058400
Δeut-339(T-POP)eutS–eutPTT2058602
Δeut-340(T-POP)eutS–eutETT2058700
Δeut-302, Δeut-337(T-POP)eutD–eutM,eutJ–eutKTT2058900
Δeut-333 Δeut-337 (T-POP)eutP–eutD,eutJ–eutKTT2058800
  • a Class 1 deletions are in-frame deletions derived from T-POP insertions. They retain no Tn10material and show no polar effect on downstream eut genes. Class 2 deletions were constructed by recombination between T-POP insertions in different eut genes. They carry a T-POP insertion at the deletion junction point and are strongly polar but express distal eut genes in the presence of tetracycline.

  • b Growth responses are scored as follows: 0, no growth; 1, very weak; 2, intermediate; 3, like wild type. Strains that express EutE but not EutBC (lyase) are listed as 0; they show extremely weak (EutE-dependent) growth that is due to a secondary minor route for conversion of ethanolamine to acetaldehyde (see Discussion). Tc, tetracycline.

  • c Strains that lack the eutBC(lyase) gene but still express the eutE gene are scored here as 0; they show extremely weak but reliably scorable growth that is due to a minor (EutE-dependent) secondary pathway of ethanolamine degradation.