Table 7.

Aerobic phenotypes of eut deletions

ClassaDeletionRemovedStrainGrowthb
−Tc+Tc
1 eut+ (Tcr)TT134403
Del1955 All eut TT206060
Δeut-300 eutJ–eutG TT191873
Δeut-301 eutG–eutH TT191883
Δeut-302 eutD–eutM TT191893
Δeut0333 eutP–eutD TT205823
2 eut +(Tcr)TT1344033
Δeut-338 (T-POP) ΔeutE–eutK TT205850c 0
Δeut–335(T-POP) ΔeutA–eutK TT2058200
Δeut-336(T-POP) eutS–eutK TT2058300
Δeut-337(T-POP) eutJ–eutK TT2058400
Δeut-339(T-POP) eutS–eutP TT2058602
Δeut-340(T-POP) eutS–eutE TT2058700
Δeut-302, Δeut-337(T-POP) eutD–eutM,eutJ–eutK TT2058900
Δeut-333 Δeut-337 (T-POP) eutP–eutD,eutJ–eutK TT2058800
  • a Class 1 deletions are in-frame deletions derived from T-POP insertions. They retain no Tn10material and show no polar effect on downstream eut genes. Class 2 deletions were constructed by recombination between T-POP insertions in different eut genes. They carry a T-POP insertion at the deletion junction point and are strongly polar but express distal eut genes in the presence of tetracycline.

  • b Growth responses are scored as follows: 0, no growth; 1, very weak; 2, intermediate; 3, like wild type. Strains that express EutE but not EutBC (lyase) are listed as 0; they show extremely weak (EutE-dependent) growth that is due to a secondary minor route for conversion of ethanolamine to acetaldehyde (see Discussion). Tc, tetracycline.

  • c Strains that lack the eutBC(lyase) gene but still express the eutE gene are scored here as 0; they show extremely weak but reliably scorable growth that is due to a minor (EutE-dependent) secondary pathway of ethanolamine degradation.