Table 1.

Strains and plasmids used

Strain or plasmidGenotype or descriptionReference, source, or construction
B. subtilis
 PY79Prototrophic26
 JH642trpC2 pheA1Lab stock
 HB4035sigDΔ::KanrJ. Helmann
 RL102spo0HΔHindIII-EcoRI::cat trpC2R. Losick
 PM126sigBΔ::catR. Losick
 AG518abr::Tn917 trpC2 pheA1A. D. Grossman
 AG839Δabr::cat trpC2 pheA1A. D. Grossman
 AG1242spo0AΔPstrpC2 pheA1A. D. Grossman
 AGS301amyE::yqxM-lacZPY79 × pAGS43
 AGS303amyE::tasA-lacZPY79 × pAGS42
 AGS305spo0HΔHindIII-EcoRI::cat::SpecramyE::tasA-lacZAGS232 × pAGS42
 AGS306sigDΔ::KanrPY79 × DNA HB4035
 AGS307sipWΩpLGW201 (sipW-lacZ)PY79 × 8G5::pLGW201 (22)
 AGS313spo0AΔ::ErmramyE::tasA-lacZAGS303 × DNA RL891 (10)
 AGS340amyE::pAGS51PY79 × pAGS51
 AGS347abrB::Tn917PY79 × DNA AG518
 AGS348ΔabrB::catPY79 × DNA AG839
 AGS349spo0HΔHindIII-EcoRI::cat::SpecrabrB::Tn917AGS232 × DNA AG518
 AGS350spo0HΔHindIII-EcoRI::cat::SpecrΔabrB::catAGS232 × DNA AG839
 AGS351abrB::Tn917 amyE::tasA-lacZAGS347 × DNA AGS303
 AGS352abrB::Tn917 spo0HΔHindIII-EcoRI::cat::SpecramyE::tasA-lacZAGS349 × DNA AGS303
 AGS361spo0A::pSPC101amyE::tasA-lacZAGS303 × DNA JKH75 (4)
 AGS362spo0A::pSPC101-S233PamyE::tasA-lacZAGS303 × DNA JKH104 (4)
 AGS363spo0A::pSPC101-G227RamyE::tasA-lacZAGS303 × DNA JKH74 (4)
 AGS364spo0A::pSPC101-F236SamyE::tasA-lacZAGS303 × DNA JKH105 (4)
 AGS365spo0A::pSPC101-V240AamyE::tasA-lacZAGS303 × DNA JKH106 (4)
 AGS366spo0A::pSPC101-V240G K265RamyE::tasA-lacZAGS303 × DNA JKH107 (4)
 AGS367spo0AΔPsamyE::tasA-lacZ trpC2 pheAAG1242 × DNA AGS303
 AGS368ΔsigB::catPY79 × DNA PM126
 AGS376amyE::pAGS53PY79 × pAGS53
 AGS377amyE::pAGS54PY79 × pAGS54
 AGS378amyE::pAGS55PY79 × pAGS55
 AGS379amyE::pAGS56PY79 × pAGS56
 AGS380amyE::pAGS57PY79 × pAGS57
 AGS381amyE::pAGS58PY79 × pAGS58
 AGS387amyE::tasA-lacZ trpC2 pheA1AGS351 × DNA AGS303
 AGS392abrB::Tn917 spo0AΔ::pSPC101amyE::tasA-lacZAGS351 × DNA JKH75 (4)
E. coli DH5αCloning hostLab stock
Plasmid
 pAGS42351 bp upstream of yqxM sipW tasA-lacZThis study
 pAGS43yqxM-lacZThis study
 pAGS51Promoterless yqxM sipW tasA-lacZThis study
 pAGS53281 bp upstream of yqxM sipW tasA-lacZThis study
 pAGS54264 bp upstream ofyqxM sipW tasA-lacZThis study
 pAGS55233 bp upstream of yqxM sipW tasA-lacZThis study
 pAGS56114 bp upstream of yqxM sipW tasA-lacZThis study
 pAGS5740 bp upstream ofyqxM sipW tasA-lacZThis study
 pAGS58351 bp upstream of yqxM sipW tasA-lacZThis study
 pDG268Permits marker replacement of lacZ fusion atamy6
 pDG364Permits marker replacement at amy7
 pSL1180Harbors extensive multiple cloning sitePharmacia