Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidDescriptionReference or source
Streptomyces spp.
S. cinnamonensisA3823.5Monensin-overproducing strainEli Lilly & Co.
S. lividansTK64pro-2 str-69
E. coli ET12567hsdS dam-3 dcm-614
Plasmids
 pUC1817
 pGM160Temperature-sensitive plasmid16
 pIJ963Contains the hygBgene13
 pOCI403Contains 2.3-kbBamHI fragment of mutB in pUC18This work
 pOCI352Hygromycin resistance gene cloned asBamHI-BglII fragment in the BglII site of pOCI403This work
 pOCI353Contains the disruptedmutB gene cloned as BamHI fragment in pGM160This work