Table 2.

Kinetic parameters for PrpC citrate synthase and 2-methylcitrate synthase activity

Parameter Valuea
Citrate synthase activity
Km acetyl-CoA285 ± 20 μM
Km oxaloacetate14 ± 4 μM
V max 2 ± 0.1 μmol/min/mg
k cat 1.4 ± 0.1b s−1
k cat/Km acetyl-CoA5 × 103 M−1 s−1
2-Methylcitrate synthase activity
Km propionyl-CoA48 ± 8 μM
Km oxaloacetate12 ± 2 μM
V max 10 ± 0.3 μmol/min/mg
k cat 7.4 ± 0.2b s−1
k cat/Km propionyl-CoA150 × 103 M−1 s−1
  • a Values reported as mean ± standard deviation.

  • b k cat values were calculated per subunit of His-tagged PrpC.