Table 1.

Strains used in this study

StrainGenotypeReference or source
E. coli
 DH5α/F′F′/endA1 hsdR17 (rk mk +)supE44 thi-1 recA1 gyrA (Nalr) relA1Δ(lacZYA-argF)U169 deoR[φ80dlacΔ(lacZ)M15]New England Biolabs
 BL21(λDE3)F ompT hsdSB (rB mB +) dcm gal λ(DE3)Novagen
 JE4570BL21(λDE3)/pPRP62 (T7 rpo+prpC+ bla+ )This work
S. typhimurium
 JR501 hsdSA29 hsdSB121 hsdL6 metA22 metE551 trpC2 ilv-452 rpsL120 xyl-404 galE719 H1-b H2-en,n,x (Fels2) fla-66 nml 23
 MST226 aceA101::Tn10 S. Maloy
 MST229 aceB102::Tn10 S. Maloy
 MST2026 aceK::Tn10 S. Maloy
 RT906 pps-81::MudI1734(kan+ )R. Jeter
 RT910 pps-85::Tn10Δ16Δ17 R. Jeter
 TR6583 metE205 ara-9 K. Sanderson via J. Roth
 Derivatives of TR6583
  JE3907 prpC167 zai-6386::Tn10Δ16Δ17 12
  JE3909 prpD169 zai-6386::Tn10Δ16Δ17 12
  JE3913 prpC173 zai-6386::Tn10Δ16Δ17 12
  JE3914 prpD174 zai-6386::Tn10Δ16Δ17 12
  JE3946 prpB195 zai-6386::Tn10Δ16Δ17 12
  JE3961 prpB210 zai-6386::Tn10Δ16Δ17 12
  JE4184pGP1-2 (T7 rpo+kan+ ), pPRP38 (prpE+bla+ ) 13
  JE4287pGP1-2 (T7 rpo+kan+ ), pT7-6 (bla+ ) 13
  JE4330pGP1-2 (T7 rpo+kan+ ), pPRP22 (prpC+bla+ ) 12