Table 2.

Flagellar morphology in various mutants under conditions of overproduction and underproduction of the hook protein FlgE

Host strain and genotypePlasmidFlagellar morphologya
FlgE overproductionb
 SJW1103(wt)None Normal hook and filament
 SJW1103(wt)pKM1500 Normal hook and filament
 SJW1353(flgE)pKM1500 Normal hook and filament
 SJW6017(ΔfliC)pKM1500 Normal hook; no filament
 SJW2149(fliD)pKM1500 Normal hook; no filament
 SJW2172(flgL)pKM1500 Normal hook; no filament. Polyhook; no filament
 SJW2177(flgK)pKM1500 Normal hook and polyhook; no filament. Superpolyhook; no filament
 SJW108(fliK)pKM1500 Superpolyhook; no filament
 MY3018(fliK flhB)pKM1500 Polyhook; normal filament
 SJW1469(flgH)pKM1500 Basal body lacking L ring; normal hook; no filament. Basal body lacking L ring; polyhook; filament.
 SJW1351(flgI) or SJW1446(flgA)pKM1500 Basal body lacking L and P rings; normal hook; no filament. Basal body lacking L and P rings; polyhook; filament.
FlgE underproductionc
 SJW1103(wt)None Normal hook and filament
 SJW1353(flgE)pKM1501 Normal hook and filament. Normal hook; no filament
 SJW1353(flgE)pKM1508 Normal hook; no filament. No hook or filament
 SJW1353(flgE)pKM1505 No hook or filament
  • a The predominant morphology is shown in boldface.

  • b pKM1500 is a pTrc99A-based plasmid carrying the wild-type flgE gene.

  • c pKM1501, pKM1508, and pKM1505 are pTrc99A-based, pET22b-based, and pBR322-based plasmids, respectively, all carrying the W208opal allele of the flgE gene.