Table 3.

Comparison of osmoregulated fusion expression in attached and free-living bacteria grown under standard conditions or under anaerobiosis

FusionCulture conditionsaβ-Galactosidase sp act (U/mg protein)b
23 h45 h
Free bacteriaAttached bacteriaFree bacteriaAttached bacteria
f92-lacZ Standard16.8 ± 3.610.8 ± 0.920.0 ± 5.011.0 ± 0.4
AnaerobiosisNDc ND15.0 ± 3.77.8 ± 0.9
ompC-lacZ Standard475 ± 51,498 ± 194454 ± 341,497 ± 114
Anaerobiosis614 ± 11,340 ± 50644 ± 511,530 ± 67
proU-lacZ Standard7.9 ± 1.721.9 ± 4.921.0 ± 1.632.5 ± 4.7
Anaerobiosis05.0 ± 2.4NDND
  • a Biofilm cultures were performed in M63-mannitol medium at 30°C in petri dishes incubated in anaerobic jars (anaerobiosis) or not (standard).

  • b Results are means ± standard deviations of three determinations.

  • c ND, not determined.