Table 1.

Strains, primers, and plasmids used in this study

Strain, primer, or plasmidGenotype, sequence, or description
S. cerevisiaeASY240 ura3-1 leu2-3 112 his3-11,15 trp1Δ63 ade2-1 ade3::hisG
ras1::HIS3 ras2::LEU2 (pAS1076)
C. albicans
RAS1/ras1-1 RAS1/ras1Δ::hisG-URA3-hisG
RAS1/ras1-2 RAS1/ras1Δ::hisG
ras1-2/ras1-3 ras1Δ::hisG/ras1Δ::hph-URA3-hph
ras1-2/ras1-4 ras1Δ::hisG/ras1Δ::hph
ras1-2/ras1-4/RAS1 ras1Δ::hisG/ras1Δ::hph/ade2::RAS1-URA3
RAS1/RAS1/M-RAS1 RAS1/RAS1/ade2::Mal-RAS1-URA3
RAS1/RAS1/M-RAS1V13 RAS1/RAS1/ade2::Mal-RAS1V13-URA3
RAS1/RAS1/M-RAS1A16 RAS1/RAS1/ade2::Mal-RAS1A16-URA3
Primers
 QF61 (hphgene)5′-GGATCGATATGAAAAAGCCTGAACTCACCG-3′
 QF62 (hph gene)5′-GGATCGATCTATTCCTTTGCCCTCGG-3′
 CaRAS1-NF (5′RAS1)5′-GGTTAATGAATGAATGGCTTGC-3′
 QF92 (5′RAS1)5′-CGGGATCCGTATATGTGTGGATATGAATTTG-3′
 QF93 (3′RAS1)5′-CGGGATCCCCTATCCAATTGATTGTTTCC-3′
 QF94 (3′RAS1)5′-CTCCAGAAAGATCATTTGGTG-3′
 QF91 (RAS1)5′-TAAGCTTCATACCATGTTGAGAGAATAT-3′
 QF87 (RAS1V13 )5′-TTGTTGTTGGAGGTGTTGGTGTTGGTAAAT-3′
 QF88 (RAS1V13 )5′-ATTTACCAACACCAACACCTCCAACAACAA-3′
 QF89 (RAS1A16 )5′-GAGGTGGTGGTGTTGCTAAATCCGCTTTAAC-3′
 QF90 (RAS1A16 )5′-GTTAAAGCGGATTTAGCAACACCACCACCTC-3′
Plasmids
 pAS1076Human v-RAS gene under Adh promoter in pACYC184 with TRP1-ARS1 from YRP7
 R2Cla RAS1 ClaI fragment in pBluescript II KS(+)
 pQF137 RAS1 deletion construct withhisG-URA3-hisG cassette
 pQF138 RAS1deletion construct with hph-URA3-hph cassette
 BES116 ADE2-URA3-ADE2 AscI fragment in pBluescript II KS(+)
 BES119 ADE2-URA3-pMAL2-ADE2 AscI fragment in pBluescript II KS(+)
 pQF119 RAS1 in BES116
 pQF144.1 RAS1 in BES119
 pQF145.2 RAS1V13 in BES119
 pQF146.1 RAS1A16 in BES119