Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidGenotype or relevant characteristicsSource or reference
C. jejuni
  NCTC 11168Parental strainNational Collection of Type Cultures
  AV6311168perR::KanrThis study
  AV6711168 perR::Kanrfur::CmrThis study
E. coli DH5αFφ80dlacZΔM15Gibco BRL
 pBluescript II SK−Cloning vector, AprStratagene
 pJMK30C. colikanamycin (Kanr) resistance cassette in pUC1931
 pAV2051.4-kb PCR-amplified fragment of C. jejuni NCTC 11168 containing theperR region in pBluescriptThis study
 pAV214SmaI-cut Kanr cassette of pJMK30 cloned in the EcoRV site in perR in pAV205; the Kanr cassette is in the reverse orientation with respect toperRThis study