Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidGenotype or relevant characteristicsSource or reference
C. jejuni
  NCTC 11168Parental strainNational Collection of Type Cultures
  AV6311168perR::Kanr This study
  AV4211168fur::Cmr 31
  AV6711168 perR::Kanr fur::Cmr This study
E. coli DH5αFφ80dlacZΔM15Gibco BRL
 pBluescript II SK−Cloning vector, Apr Stratagene
 pJMK30 C. colikanamycin (Kanr) resistance cassette in pUC19 31
 pAV2051.4-kb PCR-amplified fragment of C. jejuni NCTC 11168 containing theperR region in pBluescriptThis study
 pAV214 SmaI-cut Kanr cassette of pJMK30 cloned in the EcoRV site in perR in pAV205; the Kanr cassette is in the reverse orientation with respect toperR This study