Table 2.

Molar ratios of the noncarbohydrate substituents in the isolated oligosaccharidesa

SampleRatios of substituentsb
Pyr (1.46 ppm)Ac (2.15 ppm)Suc (2.63 to 2.70 ppm)
M110.68 (0.68)c 0 (0)d
M210.67 (0.67)1 (1)
M310.71 (0.71)2.1 (2)
D121.29 (0.65)0.85 (1)
D221.28 (0.64)1.8 (2)
D321.33 (0.66)2.9 (3)
D421.37 (0.69)3.9 (4)
T132.1 (0.70)1.1 (1)
T232.0 (0.70)1.7 (2)
T331.96 (0.65)2.9 (3)
T432.0 (0.66)3.7 (4)
T532.0 (0.66)4.8 (5)
T632.0 (0.66)5.9 (6)
  • a The ratios were determined from the integration of the acetyl, pyruvyl, and succinyl signals in the one-dimentional 1H-NMR spectra of the isolated succinoglycan oligosaccharides. The 1H-NMR spectra were recorded in D2O at 70°C.

  • b Pyr, pyruvyl; Ac, acetyl; Suc, succinyl.

  • c Numbers in the parentheses are the average degree of acetylation per subunit.

  • d Numbers in the parentheses are the numbers of succinyl groups per molecule.