Table 1.

E. coli strains and plasmids used in this study

Strain or plasmidRelevant characteristics or genotypeReference or source
Strains
 BW21355K-12 F rph-1Δ(lac)X74 12
 AE2293K-12 F metB+ glpK1 cpxA9 31
 JP466MC4100 Δfrd-101 F′ pOXgenargE::Tn10 30
 ECL3500BW21355argE::Tn10 This study
 ECL1212MC4100 Δfrd-101 F′ pOXgen Δ(cpxRA)2 29
 ECL3501BW21355 Δ(cpxRA)2 This study
 ECL3502ECL3501 Φ(cpxR+A+-lacZ)This study
 ECL3503ECL3501 Φ(cpxRΔA+-lacZ)This study
 ECL3504ECL3501 Φ(cpxR+A *-lacZ)This study
 ECL3505ECL3501 Φ(cpxRΔA *-lacZ)This study
 GS015K-12rpoS::Tn10 1
 ECL3506ECL3502rpoS::Tn10 This study
 ECL3507ECL3503rpoS::Tn10 This study
Plasmids
 pRS415 lac-based promoter fusion vector 37
 pRS415/R+A+ pRS415 containing the cpxR+A+ operonThis study
 pRS415/RΔA+ pRS415 containing the cpxRΔA+ operonThis study
 pRS415/R+A * pRS415 containing the cpxR+A * operonThis study
 pRS415/RΔA * pRS415 containing thecpxRΔA * operonThis study
 pAlter-1Cloning vectorPromega
 pAlter/R+A+ pAlter-1 containing the cpxR+A+ operonThis study
 pAlter/RΔA+ pAlter-1 containing the cpxRΔA+ operonThis study
 pAlter/R+A * pAlter-1 containing thecpxR+A * operonThis study
 pAlter/RΔA * pAlter-1 containing the cpxRΔA * operonThis study