Table 1.

Strains and plasmids

Strain or Plasmid (species)DescriptionaReference, source, or construction
Strains
 PY79 (B. subtilis)Wild type 19
 AG518 (B. subtilis) abr::Tn917 trpC2 pheA1 A. D. Grossman
 AG839 (B. subtilis)Δabr::cat trpC2 pheA1 A. D. Grossman
 AGS157 (B. subtilis) sipWΔ::neo 13
 AGS175 (B. subtilis)yqxM::neo→ 13
 AGS339 (B. subtilis) yqxMΩpAGS52This study
 AGS347 (B. subtilis) abrB::Tn917 PY79 × DNA AG518
 AGS348 (B. subtilis)ΔabrB::cat PY79 × DNA AG839
 AGS354 (B. subtilis) yqxMΩpAGS52 ΔsipW::neo→ AGS339 × pAGS17-2
 AGS40 (E. coli)BL21(DE3)/pET24bThis study
 AGS249 (E. coli)BL21(DE3)/pAGS36This study
 AGS253 (E. coli)BL21(DE3)/pAGS05This study
 AGS275 (E. coli)BL21(DE3)/pAGS41This study
 AGS406 (E. coli)BL21(DE3)/pAGS65This study
 DH5α (E. coli)Cloning hostLab collection
 BL21(DE3) (E. coli)Overproduction hostLab collection
Plasmids
 pAG58Pspacexpression plasmid 6
 pAGS05Overexpression plasmid 13
 pAGS17-2Insertion deletion insipW 13
 pAGS36T7 control of proless yqxM This study
 pAGS41T7 control ofyqxM This study
 pAGS52Pspac control ofyqxM This study
 pAGS65T7 control ofyqxM and sipW This study
 pET24bOverexpression plasmidNovagen
  • a Arrows above gene names indicate directions of transcription.