Table 3.

Purification of subtilosin A

Purification stepVol (ml)Total protein (μg)AUaRecovery (%)Fold purification
TotalSpecific (AU/μg)
Culture supernatant25013,0005,0000.381001
Fractions from High Q column60NDb 1,600ND32ND
Butanol extraction154001,0242.5620.56.7
Suspension after LH-20 chromatography0.42406402.712.87.1
  • a AU, arbitrary units.

  • b ND, not done.