Table 3.

Presence of the different i6A derivatives in the tRNA of S. typhimurium carrying wild-type and mutantmiaB alleles

StrainRelevant genotypeaPlasmidGrowth temp (°C)Ratio of i6A derivativeb to m2A
i6Aio6Ams2i6Ams2io6A
GT2734 miaB2508::Tn10dCm37 0.55 0.068
GT5441 miaB2508::Tn10dCmpUST138370.0080.0060.12 0.48
GT5442 miaB2508::Tn10dCmpBluescript SK37 0.45 0.057
GT1825 miaB+ 370.0070.0070.19 0.48
410.0120.029 0.83
GT3828 miaB26 370.0600.0070.21 0.37
41 0.36 0.0080.075
GT3830 miaB27 37 0.45
41 0.42
GT3831 miaB28 37 0.44
41 0.43 0.010
GT3833 miaB29 37 0.43 0.013
41 0.41
TT2342 miaB+zbf-99::Tn10 410.0060.0100.14 0.54
GT5443 miaB26 zbf-99::Tn10 41 0.36 0.0080.0700.077
GT5444 miaB27 zbf-99::Tn10 41 0.39 0.023
GT5445 miaB28 zbf-99::Tn10 41 0.37 0.025
GT5446 miaB29 zbf-99::Tn10 41 0.44 0.018
GT5766 miaB26 zbf-99::Tn10 pUST190410.21 0.44
GT5767 miaB27 zbf-99::Tn10 pUST190410.21 0.46
GT5768 miaB28 zbf-99::Tn10 pUST190410.23 0.44
GT5769 miaB29 zbf-99::Tn10 pUST190410.19 0.45
  • a See Table 1 for complete genotypes.

  • b The i6A derivative present in the largest amount in each strain is shown in bold letters. —, not detected. Compounds present at amounts lower than 0.005 (less than 1% of the total amount of i6A-derivatives) cannot be detected.