Table 1.

Vibrio family isolates used in this study and their ability to produce acetone in the presence of l-leucine

Strain(s)dOrigin or referenceaAcetone productionb in:Verified byc
MT glucose mediumMT leucine medium
V. harveyi7OM23 41 5 (2.8)991 (1.7)HPLC, GC-MS
V. harveyi 7OM2, 7OM15, 9OM1, 9OM6, 9OM10, 9OM12 41 5–17307–2,130
V. pelagius 7OM5 41 27 (1.0)795 (0.8)
V. pelagius 9OM11 41 5 (2.6)425 (0.9)HPLC
V. splendidus D2This study3 (4.4), 17 (4.4)168 (1.7), 1,060 (2.4)HPLC
V. splendidusP5This study3–55 (3.9–6.6; n = 4)453–4,320 (1.4–2.8; n = 5)HPLC, GC-MS
V. splendidus1OM9 41 20 (3.0)577 (1.5)HPLC
V. splendidus 1OM13 41 74 (0.2)888 (0.2)
V. splendidus 33125ATCC3 (4.5)145 (1.5)HPLC
V. tubiashii 9OM7 41 7 (2.7)427 (1.7)HPLC
V. tubiashii 9SM11 41 3 (4.2)709 (1.6)
L. anguillarum 19264ATCC22 (3.2)400 (2.1)
L. pelagia 33784ATCC39 (6.2)3,130 (1.8)HPLC, GC-MS
P. angustum25915ATCC388 (4.9)3,300 (1.4)HPLC, GC-MS
P. angustum 33977ATCC208 (3.9)2,410 (1.9)
S. alga BrY 8 5 (4.2)6 (7.1)
S. amazonensis SB2B8 (3.0)23 (2.8)
S. frigidimarina ACAM 591, ACAM 600 6 10–1215–19
S. putrefaciens MR-1, MR-4 28 11–2
S. woodyi MS32 34 1 (6.3)150 (4.0)HPLC
  • a ATCC, American Type Culture Collection (Manassas, Va.).

  • b Media and the method for acetone determination are described in Materials and Methods. Levels of nutrients: glucose, 2% (wt/vol); l-leucine, 20 mM. The data shown are the averages of duplicate determinations of acetone levels (micromolar) divided by the OD600 of the culture. For experiments with individual strains the average OD600of the two cultures or range of OD600 values for multiple determinations are shown in parentheses.

  • c Method used to verify the identity of acetone produced in the culture.

  • d Abbreviations for genera: V.,Vibrio; L., Listonella; P.,Photobacterium; S., Shewanella.