Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant genotypeaReference or source
Strains
 Nissle 1917fimB7
 PC31gal tonA phx argF rel19
 HB101/F′F′ lacI Tn5 derivative of HB1018
 BOD11Nissle 1917 ΔfimEThis study
 BOD25Nissle 1917 ΔfimBEThis study
Plasmids
 pBSO8fimE from Nissle 1917 (B)This study
 pBSO10fimB from Nissle 1917 (U)This study
 pBSO11fimB′ and fimE from Nissle 1917 (U)This study
 pBSO13Phase switch from Nissle 1917 (U)This study
 pBSO21Insert′ and fimB′ from Nissle 1917 (B)This study
 pBSO22Insert′ fimB′ phase switch and fimAIC from Nissle 1917 (B)This study
 pBSO25Insert′ fimB′ phase switch andfimAIC from Nissle 1917 (M)This study
 pBSO26fimE from Fim-positive Nissle 1917 colony (U)This study
 pBSO28Phase switch from Fim-positive Nissle 1917 colony (U)This study
 pBSO31Phase switch from Fim-positive BOD11 colony (U)This study
 pBSO36Region upstream of fimB from Nissle 1917 (B)This study
 pBSO37Region upstream of fimB, phase switch, andfimAIC from Nissle 1917 (B)This study
 pBSO38Region upstream of fimB, phase switch, andfimAIC from Nissle 1917 (M)This study
 pMAK700oriTTs origin9a
 pPKL4All fim genes (B)19
 pPKL9fimB (B)16
  • a U, pUC19 based; B, pBR322 based; M, pMAK700oriT based.