Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant genotypeaReference or source
Strains
 Nissle 1917 fimB 7
 PC31 gal tonA phx argF rel 19
 HB101/F′F′ lacI Tn5 derivative of HB101 8
 BOD11Nissle 1917 ΔfimE This study
 BOD25Nissle 1917 ΔfimBE This study
Plasmids
 pBSO8 fimE from Nissle 1917 (B)This study
 pBSO10 fimB from Nissle 1917 (U)This study
 pBSO11 fimB′ and fimE from Nissle 1917 (U)This study
 pBSO13Phase switch from Nissle 1917 (U)This study
 pBSO21Insert′ and fimB′ from Nissle 1917 (B)This study
 pBSO22Insert′ fimB′ phase switch and fimAIC from Nissle 1917 (B)This study
 pBSO25Insert′ fimB′ phase switch andfimAIC from Nissle 1917 (M)This study
 pBSO26 fimE from Fim-positive Nissle 1917 colony (U)This study
 pBSO28Phase switch from Fim-positive Nissle 1917 colony (U)This study
 pBSO31Phase switch from Fim-positive BOD11 colony (U)This study
 pBSO36Region upstream of fimB from Nissle 1917 (B)This study
 pBSO37Region upstream of fimB, phase switch, andfimAIC from Nissle 1917 (B)This study
 pBSO38Region upstream of fimB, phase switch, andfimAIC from Nissle 1917 (M)This study
 pMAK700oriTTs origin 9a
 pPKL4All fim genes (B) 19
 pPKL9 fimB (B) 16
  • a U, pUC19 based; B, pBR322 based; M, pMAK700oriT based.