Table 4.

RNase III dependence of β-galactosidase activity specified by different Φ(adhE-lacZ) fusion constructsa

StrainRelevant genotypeβ-Galactosidase activity (Miller units)
ECL4012Φ(adhE-lacZ)656protein fusion9,000 ± 360
ECL4016Φ(adhE-lacZ)656 protein fusion; rnc::Tn10 620 ± 60
ECL4013Φ(adhE-lacZ)656operon fusion9,900 ± 280
ECL4017Φ(adhE-lacZ)656operon fusion;rnc::Tn10 9,800 ± 150
ECL4010Φ(adhE-lacZ)291 protein fusion3,400 ± 110
ECL4018Φ(adhE-lacZ)291 protein fusion; rnc::Tn10 3,500 ± 150
  • a The cells were grown anaerobically on LB-glucose medium and assayed in the exponential phase of growth (OD600, 0.4).