Table 5.

Complementation of strain ECL3999 (adhE::kan) with plasmids bearing different constructs of adhE

PlasmidRelevant characteristicsNo. of colonies testeda
LB Ap (aerobic)MM glucose (anaerobic)
pBR322No insert5000
pJMADH1 adhE + regulatory region up to −656500496b
pJMADH2 adhE + regulatory region up to −291500491c
pJMADH3 adhE + regulatory region up to −656 but Pribnow TACAAT box (from positions −304 to −298) changed to CGGGCC5002d
  • a LB Ap, LB medium plus ampicillin; MM glucose, glucose minimal medium.

  • b Colonies were scored after 24 h of incubation at 37°C.

  • c Colonies were scored after 48 h of incubation at 37°C.

  • d Colonies were scored after 72 h of incubation at 37°C.