Table 2.

Effects of crc-24 mutation on levels of carbohydrate catabolism enzymes in P. aeruginosa PA14

GenotypeAdditionaAvg activity ± SDb
Gluc-6-P DHMannitol DHNAD-specific Gly-3-P DHNADP-specific Gly-3-P DHc
crc + None256 ± 23d 56 ± 111,014 ± 102130 ± 67
Succinate32 ± 515 ± 1.4125 ± 576 ± 4
crc-24 None335 ± 70100 ± 131,203 ± 103168 ± 8
Succinate191 ± 4873 ± 11755 ± 67130 ± 20
  • a Bacteria were grown on minimal medium with 30 mM mannitol with or without the addition of 40 mM succinate. See Materials and Methods for details.

  • b Abbreviations: Gluc-6-P DH, glucose 6-phosphate dehydrogenase; Gly-3-P DH, glyceraldehyde 3-phosphate dehydrogenase.

  • c The level of NADP-specific glyceraldehyde 3-phosphate dehydrogenase activity is Crc independent and served as a control.

  • d The enzyme assays monitored the reduction of NAD(P) to NAD(P)H, and specific activity is expressed as nanomoles of NAD(P)H produced per minute per milligram of protein. The data presented are for duplicate (NAD- and NADP-dependent glyceraldehyde 3-phosphate dehydrogenase) or triplicate (glucose 6-phosphate dehydrogenase and mannitol dehydrogenase) assays.