Table 1.

Bacillus subtilis strains used in this study

StrainaRelevant genotypeSource or reference(s)b
JH642trpC2 phe-1Laboratory stock
JH703spo0AΔ204Laboratory stock
JH12474Δspo0H::catBH-1→JH642;10
JH12546spo0A12 abrB::Tn917::mls23a
JH12575abrB::Tn917::mls23a
JH12834rapA::Tn917::mls26
JH12954ΔphrA::cat28
JH11125rapE::catpSK28→JH642
JH11435spo0FY13S26
JH11450ΔphrE::catpSK34→JH642
JH11455ΔphrE::ermpCm::Erm→JH11450;34
JH11499ΔphrA::cat ΔphrE::ermJH12954→JH11455
JH11505amyE::(rapE-lacZ cat)pSK35→JH642
JH11506amyE::(phrE-lacZ cat)pSK36→JH642
JH11534rapE::tetpCm::Tet→JH11125;34
JH11542rapE::tet phrE::catpSK34→JH11534
JH11751rapA::Tn917::mls rapE::catJH12834→JH11125
JH11760spo0FY13S ΔphrE::catpSK34→JH11435
JH22141comA::cat amyE::(rapE-lacZ spc)BD1626→JH11505 Spc;8c
JH22143spo0A Δ204 amyE::(rapE-lacZ spc)11505 Spc→JH703c
JH22144comA::cat amyE::(phrE-lacZ spc)BD1626→JH11506 Spcc
JH22145spo0H::cat amyE::(phrE-lacZ spc)JH12474→JH11506 Spcc
JH22146spo0A Δ204 amyE::(phrE-lacZ spc)JH11506 Spc→JH703;8
JH22152spo0A Δ204 abrB::Tn917::mls amyE::(rapE-lacZ spc)JH12575→JH22143
JH22154spo0A Δ204 abrB::Tn917::mls amyE::(phrE-lacZ spc)JH12575→JH22146
  • a All JH strains are derivatives of JH642 and therefore carry the trpC2 phe-1 auxotrophic markers.

  • b →, construction by transformation.

  • c These strains were obtained by replacement of the chloramphenicol resistance gene with the spectinomycin resistance gene by means of plasmid pCm::Spc (34).