Table 3.

Sporulation efficiency of rap and/orphr mutant strainsa

StrainbRelevant phenotypeViable cell countSpore count% of sporulationc
JH642WT2.3 × 1081.35 × 10858.7
JH11450PhrE4.8 × 1081.1 × 10822.9
JH11542RapEPhrE2.2 × 1081.5 × 10868.2
JH11125RapE2.3 × 1081.5 × 10865.2
JH11435Spo0F (Y13S)1.5 × 1081.3 × 10886.6
JH11760Spo0F (Y13S) PhrE1.2 × 1081.0 × 10883.3
JH12954PhrA4.4 × 1084.0 × 1079.1
JH11499PhrAPhrE4.9 × 1083.6 × 1077.3
JH12834RapA1.8 × 1081.4 × 10877.7
JH11751RapARapE1.6 × 1081.2 × 10875.0
  • a Representative of three independent experiments. WT, wild type.

  • b Strains were grown for 30 h at 37°C in Schaeffer's sporulation medium.

  • c Percentage of sporulation is expressed as the ratio between spore counts and viable cell counts. The significant differences observed in percentages, despite the limited differences in absolute spore counts, are due to the highly reproducible differences in viable counts between sporulating and nonsporulating cells.