Table 1.

Kinetic properties of HpaC

Kinetic parameter and substrateValue
Km (μM)
 FMNa 2.1
 Riboflavina 2.6
 FADa 3.1
 NADHb 40
V max (μmol/min/mg)
 FMNa 70
 Riboflavina 59
 FADa 38
 FMNc 0.5
 Riboflavinc 0.4
 FADc 0.1
 Cytochromecd 1.8
 Fe3+d 12.4
  • a Km andV max were obtained by using 150 μM NADH as the electron donor.

  • b Km was obtained by using 60 μM FMN as the electron acceptor.

  • c V max was obtained by using 150 μM NADPH as the electron donor.

  • d V max was obtained by using 150 μM NADH and 60 μM FMN.