Table 2.

Purification scheme of the DNA polymerase from‚ÄČP.‚ÄČislandicum

Purification stepTotal protein (mg)Total activity (U)Sp act (U/mg)Purification (fold)Recovery (%)
Crude extract45.01,13825.31100
Heat denaturation11.576866.82.668
Streptactin chromatography0.45021,255.049.644