Table 1.

Bacterial strains and plasmids

Strain or plasmidGenotype or relevant characteristic(s)aSource or reference
Strains
S. aureus
 ISP7948325pig-131 34
 MT231428325 pig-131 flqB 24
 BF158325 pig-131 flqB arlS::Tn917LTV1This study
 RN42208325-4 r 18
 KLE820RN4220norA::cat 14
 RN63908325-4 Hla+Prt+ 26
 RN6911RN6390agr::tetM 27
 ALC136RN6390sar::Tn917LTV1 8
 ALC135RN6390 agr::tetM sar::Tn917LTV1 7
E. coli DH5αF-φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 phoA hsdR17(rK mK )supE44O λ thi-1 gyrA96 relA1 Gibco BRL
Plasmids
 pGEM3-zf(+)2.9-kb E. colicloning vector, Apr Promega
 pGB24.3-kbE. coli cloning vector, Smr 9
 pWN201810.5-kbS. aureus promoter-probe vector, Cmr 39
 pE1943.7-kb S. aureus cloning vector, Emr 33
 pSK2653-kb S. aureus cloning vector, Cmr 32
 pBF8-30315 bp containing the entire promoter of norA cloned upstream of the blaZ gene of pWN2018This study
 pBF3-50250 bp containing a truncated promoter of norA cloned upstream of the blaZ gene of pWN2018This study
 pBF4-3130 bp containing a truncated promoter ofnorA cloned upstream of the blaZ gene of pWN2018This study
 pBF15-5110 bp containing a truncated promoter of norA cloned upstream of the blaZ gene of pWN2018This study
 pBF16-42.4-kb PCR product containing the arlR-arlS locus cloned into pGB2 + pE194This study
 pBF172.4-kb PCR product containing the arlR-arlSlocus cloned into pGB2 + pSK265This study
  • a Resistance determinants: Ap, ampicillin; Sm, streptomycin; Cm, chloramphenicol; Em, erythromycin.